No, but re-running doesn't hurt (but doesn't help, either). The Quadro Tweak Utility puts information into nVidia's section of the registry where eCabinet Systems installs and upgrades don't write.
Curiously, why do you ask?
Dean Fehribach
I.S. Mgr., Thermwood
Dell Workstation T1650 / XEON E3 / 8GB RAM / 1GB nVidia Quadro 600 / Windows 8 Pro x64
I ask because I am being VERY cautious and will do ONLY what is recommended, after someone has tried it out. I did not want to run the utility after installing Build 3 if it was going to cause unexpected or unsuspected problems, Dean.